mouse anti ova ige standards  (Bio-Rad)

Journal: The World Allergy Organization Journal

Article Title: High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias ☆

doi: 10.1016/j.waojou.2025.101031

Figure Lengend Snippet: Effect of feeding mice for 7 weeks either normal diet or high protein diet on serum levels of (A) OVA-specific IgE (B) OVA-specific IgG1 and (C) OVA-specific IgG2a from OVA-sensitized mice (n = 11 in each group) after OVA intranasal challenge. The high protein diet group showed higher levels of both OVA-specific IgE and IgG1compared to the normal diet group. ns: not significant, CI: confidence interval. IQR: interquartile range.

Article Snippet: After washing the wells, blocking buffer (0.5% gelatin in wash buffer or 3% Rat serum in wash buffer) was added and the plates incubated for 2 h. Appropriate reference standard OVA-IgE (Chondrex #3006) or OVA-IgG1 (Chondrex # 7093) or OVA-IgG2a (Chondrex # 7095) and diluted serum samples were then added to the wells and incubated for 3 h. This was followed by incubation with OVA-Biotin for 1 h and with streptavidin-HRP for 30 min. Tetramethylbenzidine substrate was added and the enzyme-substrate reaction stopped after 15 min, and the optical density measured at 450 nm.

Techniques: